Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance

Guihua Lv; Guojin Guo; Jianjian Chen; Xiangnan Li; Zhenxing Wu

doi:doi:10.11648/j.jps.20190706.15

| Peer-Reviewed

Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance

Guihua Lv, Guojin Guo, Jianjian Chen, Xiangnan Li, Zhenxing Wu

Published in Journal of Plant Sciences (Volume 7, Issue 6)

Received: 15 October 2019 Accepted: 12 November 2019 Published: 4 December 2019

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Nicosulfuron, a sulfonylurea herbicide is registered for use on field, controns many annual and perennial grass weeds. The accumulation of nicosulfuron will increase toxicity and induce unfavorable oxidative stress in plants. Acetolactate synthase (ALS, EC 2.2.1.6) and glutathione transferases (GSTs, E. C.2.5.1.18), which participate in detoxification of xenobiotics and limit oxidative damages of cellular macromolecules, are important groups of cytoprotective enzymes. This study aims to investigate the toxic effect of nicosulfuron on the detoxification enzyme activities and gene expressions in maize seedling. Specifically, Nicosulfuron-tolerant sweet inbred line ‘JP233’ and nicosulfuron-sensitive sweet inbred line ‘Z2H4’ are subjected to 80mg kg^–1 nicosulfuron treatment when the fourth leaves are fully developed, and the resulting effects are compared to those processed by water. After nicosulfuron treatment, it is found that ALS and GST enzymes activities of Z2H4 are significantly lower than those of JP233. Compared to Z2H4, nicosulfuron treatment increases the expression levels of GST1, ALS1 and ALS2 genes in JP233. These results suggest that the increased transcription level of these detoxifying enzymes might play a vital role in reducing the toxicity of nicosulfuron and the oxidative stress induced by nicosulfuron in maize seedlings. The research will improve our understanding of the function of maize detoxification enzymes and genes in herbicide metabolism.

Published in	Journal of Plant Sciences (Volume 7, Issue 6)
DOI	10.11648/j.jps.20190706.15
Page(s)	158-163
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Nicosulfuron, Sweet Corn, Acetolactate Synthase, Glutathione Transferase, Gene Expression, Oxidative Stress, Toxicity

References

[1]	Duggleby RG, McCourt JA, Guddat LW. Structure and mechanism of inhibition of plant acetohydroxyacid synthase. Plant Physiol Biochem. 2008; 46: 309–324.
[2]	Singh B, Schmitt G, Lillis M, Hand JM, Misra R. Overexpression of acetohydroxyacid synthase from arabidopsis as an inducible fusion protein in Escherichia coli: production of polyclonal antibodies, and immunological characterization of the enzyme. Plant Physiol. 1991; 97: 657-662.
[3]	Singh R, Pandey N, Naskar J, Shirke PA. Physiological performance and differential expression profiling of genes associated with drought tolerance in contrasting varieties of two Gossypium species. Protoplasma. 2015; 252 (2): 423-438.
[4]	Rey-Caballero J, Menéndez J, Osuna MD, Salas M, Torra J. Target-site and non-target-site resistance mechanisms to ALS inhibiting herbicides in Papaver rhoeas. Pestici Biochem Physiol. 2017; 138: 57-65.
[5]	Beckie HJ, Tardif FJ. Herbicide cross resistance in weeds. Crop Prot. 2012; 35: 15–28.
[6]	Yu Q, Powles SB. Resistance to AHAS inhibitor herbicides: current understanding. Pest Manag Sci. 2014; 70: 1340-1350.
[7]	Klaus K, Roberto T, Enrico M. Old enzymes for a new job: Herbicide detoxification in plants. Plant Physiol. 1996; 111: 349-353.
[8]	McGonigle B, Keeler SJ, Lau SM, Koeppe MK, O'Keefe DP. A genomics approach to the comprehensive analysis of the glutathione S-transferase gene family in soybean and maize. Plant Physiol. 2000; 124 (3): 1105-1120.
[9]	Guo XL, Tao B, Gao XW. Characteristic of maize glutathione S-transferase (GSTs) and induction action of acetochlor. J Maize Sci. 2008; 16 (1): 122–125.
[10]	Banerjee S, Goswami R. GST profile expression study in some selected plants: in silico approach. Mol Cell Biochem. 2010; 336 (1-2): 109-126.
[11]	Karavangeli M, Labrou NE, Clonis YD, Tsaftarisbc A. Development of transgenic tobaccoplants overexpressing maize glutathione S-transferase I for chloroacetanilide herbicide phytoremediation. Biomol Eng. 2005; 22 (4): 121-128.
[12]	Mimmo T, Bartucca ML, Del Buono D, Cesco S. Italian ryegrass for the phytoremediation of solutions polluted with terbuthylazine. Chemosphere. 2015; 119: 31-36.
[13]	Fan ZJ, Qian CF, Li ZM, Wang LX. Specific activity determination of acetolactate synthase from maize (Zea mays L.). In: Han W N, Zhen G Y. eds. The Proceedings of the 18th Asia-Pacific Weed Science Society Conference. 2001; 515-522. May 28-June 2, Beijing: Standards Press of China.
[14]	Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72: 248–254.
[15]	Liu XM, Xu X, Li BH, Wang XQ, Wang GQ, Li M. RNA-Seq Transcriptome analysis of maize inbred carrying nicosulfuron-tolerant and nicosulfuron-susceptible alleles. Int J Mol Sci. 2015; 16: 5975-5989.
[16]	Wiliams MM, Wax LM, Pataky JK, Meyer MD. Further evidence of a genetic basis for varied levels of injury to sweet corn hybrids from cytochrome P450-metabolized herbicides applied postemergence. HortScience. 2008; 43 (7): 2093-2097.
[17]	Hatton PJ, Dixon D, Cole DJ, Edwards R. Glutathione transferase activities and herbicide selectivity in maize and associated weed species. Pesticide Science. 1996; 46 (3): 267–275.
[18]	Sommer A, Boger P. Characterization of Recombinant Com Glutathione S-Transferase isoforms I, II, III, and IV. Pestici Biochem Physiol. 1999; 63 (3): 127-138.
[19]	Sytykiewicz H. Expression patterns of glutathione transferase gene (GstI) in maize seedlings under juglone-induced oxidative stress. Int J Mol Sci. 2011; 12 (11): 7982-7995.

Cite This Article

Plain Text BibTeX RIS

APA Style

Guihua Lv, Guojin Guo, Jianjian Chen, Xiangnan Li, Zhenxing Wu. (2019). Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance. Journal of Plant Sciences, 7(6), 158-163. https://doi.org/10.11648/j.jps.20190706.15

Copy | Download

ACS Style

Guihua Lv; Guojin Guo; Jianjian Chen; Xiangnan Li; Zhenxing Wu. Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance. J. Plant Sci. 2019, 7(6), 158-163. doi: 10.11648/j.jps.20190706.15

Copy | Download

AMA Style

Guihua Lv, Guojin Guo, Jianjian Chen, Xiangnan Li, Zhenxing Wu. Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance. J Plant Sci. 2019;7(6):158-163. doi: 10.11648/j.jps.20190706.15

Copy | Download

@article{10.11648/j.jps.20190706.15,
  author = {Guihua Lv and Guojin Guo and Jianjian Chen and Xiangnan Li and Zhenxing Wu},
  title = {Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance},
  journal = {Journal of Plant Sciences},
  volume = {7},
  number = {6},
  pages = {158-163},
  doi = {10.11648/j.jps.20190706.15},
  url = {https://doi.org/10.11648/j.jps.20190706.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20190706.15},
  abstract = {Nicosulfuron, a sulfonylurea herbicide is registered for use on field, controns many annual and perennial grass weeds. The accumulation of nicosulfuron will increase toxicity and induce unfavorable oxidative stress in plants. Acetolactate synthase (ALS, EC 2.2.1.6) and glutathione transferases (GSTs, E. C.2.5.1.18), which participate in detoxification of xenobiotics and limit oxidative damages of cellular macromolecules, are important groups of cytoprotective enzymes. This study aims to investigate the toxic effect of nicosulfuron on the detoxification enzyme activities and gene expressions in maize seedling. Specifically, Nicosulfuron-tolerant sweet inbred line ‘JP233’ and nicosulfuron-sensitive sweet inbred line ‘Z2H4’ are subjected to 80mg kg–1 nicosulfuron treatment when the fourth leaves are fully developed, and the resulting effects are compared to those processed by water. After nicosulfuron treatment, it is found that ALS and GST enzymes activities of Z2H4 are significantly lower than those of JP233. Compared to Z2H4, nicosulfuron treatment increases the expression levels of GST1, ALS1 and ALS2 genes in JP233. These results suggest that the increased transcription level of these detoxifying enzymes might play a vital role in reducing the toxicity of nicosulfuron and the oxidative stress induced by nicosulfuron in maize seedlings. The research will improve our understanding of the function of maize detoxification enzymes and genes in herbicide metabolism.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance
AU  - Guihua Lv
AU  - Guojin Guo
AU  - Jianjian Chen
AU  - Xiangnan Li
AU  - Zhenxing Wu
Y1  - 2019/12/04
PY  - 2019
N1  - https://doi.org/10.11648/j.jps.20190706.15
DO  - 10.11648/j.jps.20190706.15
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 158
EP  - 163
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20190706.15
AB  - Nicosulfuron, a sulfonylurea herbicide is registered for use on field, controns many annual and perennial grass weeds. The accumulation of nicosulfuron will increase toxicity and induce unfavorable oxidative stress in plants. Acetolactate synthase (ALS, EC 2.2.1.6) and glutathione transferases (GSTs, E. C.2.5.1.18), which participate in detoxification of xenobiotics and limit oxidative damages of cellular macromolecules, are important groups of cytoprotective enzymes. This study aims to investigate the toxic effect of nicosulfuron on the detoxification enzyme activities and gene expressions in maize seedling. Specifically, Nicosulfuron-tolerant sweet inbred line ‘JP233’ and nicosulfuron-sensitive sweet inbred line ‘Z2H4’ are subjected to 80mg kg–1 nicosulfuron treatment when the fourth leaves are fully developed, and the resulting effects are compared to those processed by water. After nicosulfuron treatment, it is found that ALS and GST enzymes activities of Z2H4 are significantly lower than those of JP233. Compared to Z2H4, nicosulfuron treatment increases the expression levels of GST1, ALS1 and ALS2 genes in JP233. These results suggest that the increased transcription level of these detoxifying enzymes might play a vital role in reducing the toxicity of nicosulfuron and the oxidative stress induced by nicosulfuron in maize seedlings. The research will improve our understanding of the function of maize detoxification enzymes and genes in herbicide metabolism.
VL  - 7
IS  - 6
ER  -

Copy | Download

Author Information

Guihua Lv

Dongyang Maize Research Institute of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Dongyang, P. R. China
Guojin Guo

Dongyang Maize Research Institute of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Dongyang, P. R. China
Jianjian Chen

Dongyang Maize Research Institute of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Dongyang, P. R. China
Xiangnan Li

Dongyang Maize Research Institute of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Dongyang, P. R. China
Zhenxing Wu

Dongyang Maize Research Institute of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Dongyang, P. R. China

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Guihua Lv, Guojin Guo, Jianjian Chen, Xiangnan Li, Zhenxing Wu. (2019). Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance. Journal of Plant Sciences, 7(6), 158-163. https://doi.org/10.11648/j.jps.20190706.15

Copy | Download

ACS Style

Guihua Lv; Guojin Guo; Jianjian Chen; Xiangnan Li; Zhenxing Wu. Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance. J. Plant Sci. 2019, 7(6), 158-163. doi: 10.11648/j.jps.20190706.15

Copy | Download

AMA Style

Guihua Lv, Guojin Guo, Jianjian Chen, Xiangnan Li, Zhenxing Wu. Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance. J Plant Sci. 2019;7(6):158-163. doi: 10.11648/j.jps.20190706.15

Copy | Download

@article{10.11648/j.jps.20190706.15,
  author = {Guihua Lv and Guojin Guo and Jianjian Chen and Xiangnan Li and Zhenxing Wu},
  title = {Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance},
  journal = {Journal of Plant Sciences},
  volume = {7},
  number = {6},
  pages = {158-163},
  doi = {10.11648/j.jps.20190706.15},
  url = {https://doi.org/10.11648/j.jps.20190706.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20190706.15},
  abstract = {Nicosulfuron, a sulfonylurea herbicide is registered for use on field, controns many annual and perennial grass weeds. The accumulation of nicosulfuron will increase toxicity and induce unfavorable oxidative stress in plants. Acetolactate synthase (ALS, EC 2.2.1.6) and glutathione transferases (GSTs, E. C.2.5.1.18), which participate in detoxification of xenobiotics and limit oxidative damages of cellular macromolecules, are important groups of cytoprotective enzymes. This study aims to investigate the toxic effect of nicosulfuron on the detoxification enzyme activities and gene expressions in maize seedling. Specifically, Nicosulfuron-tolerant sweet inbred line ‘JP233’ and nicosulfuron-sensitive sweet inbred line ‘Z2H4’ are subjected to 80mg kg–1 nicosulfuron treatment when the fourth leaves are fully developed, and the resulting effects are compared to those processed by water. After nicosulfuron treatment, it is found that ALS and GST enzymes activities of Z2H4 are significantly lower than those of JP233. Compared to Z2H4, nicosulfuron treatment increases the expression levels of GST1, ALS1 and ALS2 genes in JP233. These results suggest that the increased transcription level of these detoxifying enzymes might play a vital role in reducing the toxicity of nicosulfuron and the oxidative stress induced by nicosulfuron in maize seedlings. The research will improve our understanding of the function of maize detoxification enzymes and genes in herbicide metabolism.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Responses of Detoxification Enzymes and Genes to Nicosulfuron Stress in Two Genotypes of Sweet Corn Differing in Nicosulfuron Tolerance
AU  - Guihua Lv
AU  - Guojin Guo
AU  - Jianjian Chen
AU  - Xiangnan Li
AU  - Zhenxing Wu
Y1  - 2019/12/04
PY  - 2019
N1  - https://doi.org/10.11648/j.jps.20190706.15
DO  - 10.11648/j.jps.20190706.15
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 158
EP  - 163
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20190706.15
AB  - Nicosulfuron, a sulfonylurea herbicide is registered for use on field, controns many annual and perennial grass weeds. The accumulation of nicosulfuron will increase toxicity and induce unfavorable oxidative stress in plants. Acetolactate synthase (ALS, EC 2.2.1.6) and glutathione transferases (GSTs, E. C.2.5.1.18), which participate in detoxification of xenobiotics and limit oxidative damages of cellular macromolecules, are important groups of cytoprotective enzymes. This study aims to investigate the toxic effect of nicosulfuron on the detoxification enzyme activities and gene expressions in maize seedling. Specifically, Nicosulfuron-tolerant sweet inbred line ‘JP233’ and nicosulfuron-sensitive sweet inbred line ‘Z2H4’ are subjected to 80mg kg–1 nicosulfuron treatment when the fourth leaves are fully developed, and the resulting effects are compared to those processed by water. After nicosulfuron treatment, it is found that ALS and GST enzymes activities of Z2H4 are significantly lower than those of JP233. Compared to Z2H4, nicosulfuron treatment increases the expression levels of GST1, ALS1 and ALS2 genes in JP233. These results suggest that the increased transcription level of these detoxifying enzymes might play a vital role in reducing the toxicity of nicosulfuron and the oxidative stress induced by nicosulfuron in maize seedlings. The research will improve our understanding of the function of maize detoxification enzymes and genes in herbicide metabolism.
VL  - 7
IS  - 6
ER  -

Copy | Download